To determine which variables those are, a statistical study of the effects was carried out, considering significant effects with p-value lower than 0.1, as shown in Table 2.
Overall, the findings were similar to those in males with all deaths considered: significant positive effects of ACA and EST, but not MB.
However, when individual autosomal backgrounds were considered, significant maternal effects were only observed for the 6326 genetic background (p = 0.026 for 6326, p = 0.067 for Sudbury, two-sample Z-tests).
For quantitative analyses using QPCR and Nanostring, changes in the transcript level were considered significant if the effect between control and MO injected embryos is more than twofolds.
A two-tailed probability value of <0.05 was considered significant for main effects and for interactions.
In the study, independent variables are considered significant if their effects on unintended pregnancy are statistically significant at the 95percentnt level of significance.
When channels were considered significant, we showed the effect size (cohen's d) and 95% confidence interval (CI).
ANOVA (mixed model) was used (effects considered significant at p<0.05).
Effects of these covariates on intercept or slope were considered significant if the Wald ratio (effect estimate: standard error) was greater than 1.96, according to a z-distribution.
QTL effects were considered significant if they exceeded the 5% chromosome-wise significance threshold (pc-value < 0.05).
