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We therefore considered Replicate 2 as a 'failed' library and excluded it from subsequent analysis.
For fixed effects, we only considered replicate ID (1 10), which corresponds to the experimental vessel, given that age (all individuals had the same day of fertilization) and sex (individuals sampled in their entirety) were not relevant factors.
Fitness components of these grandchildren of stud and dud males were then measured as described below, using one of the three replicate sets of stud and dud grandchildren for each of the three fitness components considered (replicate number was the same between the stud and dud treatments for each fitness measure).
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Four QTLs present on chromosomes 6, 12, 13, and 18 were identified as significant and replicated (considered replicated if LO D>suggestivethreshold) in both Top2 inhibitors at multiple dosages.
With such criteria, several additional SNPs would have been considered replicated in our study, some of which point towards genes putatively involved in the pathophysiology of IBD.
Single pair matings involving heterozygous parents of the same sex and carrying the same arrangement for their wild-type chromosome are considered replicates (Figure 2; Table 1).
A region identified as significant in one drug-dose phenotype was considered replicated in another drug-dose phenotype if the maximum LOD score in that region surpassed the significant or suggestive LOD score threshold.
For this stage, SNPs were considered replicated if the same HUFS SNP had a p value <0.05.
To increase both, sensitivity and specificity, it is highly advisable to increase the number of considered replicates.
Fixed effects were treatment, native species and non-native species; pots were considered replicates and included in residual effects.
Streams were considered replicates and a treatment by reach interaction effect was analysed for statistically significant differences using a probability of alpha 0.10 (Table 2).
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