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Genes with an ANOVA p-value <0.05 (4 degrees of freedom) were considered regulated in response to high-fat diet and included in the analysis.
Such functions mimic the nonlinearity in gene regulation, by assuming that a critical amount of protein has to be accumulated before a gene can be considered regulated or repressed.
Genes were considered regulated if: (1) they were expressed in at least one condition (i.e., SRSF1 and/or empty vector control); (2) fold-change was ≥1.5; and (3) the unpaired t test p value between gene intensities was ≤0.05.
Gene sets were considered regulated if the GSEA p-value was<0.05 and the FDR was <0.10.
The forward and reversible reaction fluxes were considered regulated by the phosphorylation potentials [ATP]/[ADP] or [ADP]/[ATP] in the cytosol (Materials S2).
These genes were considered regulated by 323 active HSEL (Additional file 2: Table S12).
Similar(48)
Probe sets which showed at least two fold change in intensity compared to DMSO control were considered up regulated or down regulated respectively.
A threshold ratio for defining differentially expressed proteins was set based on control replicate sample; proteins with iTRAQ ratios of > = 1.2 and < = 0.83 were considered up-regulated and down-regulated, respectively.
Samples with three or more genes in a row with Z-Scores of < = - 2 or > =2 are considered down-regulated or up-regulated, respectively.
Data across the wild type series was analyzed using the Significance analysis of Microarrays (SAM) algorithm (to calculate the False Discovery Rate (FDR [49]) and genes with a ≤1% FDR and ≥3-fold change were considered Osmotic Regulated Genes (ORGs).
We considered 1,694 regulated cassette events with significant change (average probe fold-change >1.5) in the comparison between MCF7 and MCF10.
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