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All other genes give products lacking the active site and were considered non functional.
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Note that today's common usage is a narrower sense of the word 'vestige' than was employed by Chambers, whose Vestiges of the Natural History of Creation (Chambers [1844]) referred not only to what would today be considered non-functional evolutionary leftovers, but to any traces that reveal life's past.
Hence, splice sites with CVs below 70 are herein considered non-functional.
For convenience, we considered a TFBS functional if it was supported by experimental STAT3 binding data; otherwise, the TFBS was considered non-functional.
Therefore, when the P value was less than 0.05, the sequences were considered non-functional.
Most retroposons and RTEs were considered non-functional and thus regarded as "junk DNA".
Roots were considered non-functional teeth with respect to chewing ability, and therefore considered as missing teeth.
Group IV proteins were considered non-functional due to the loss of the zinc-finger domain [ 10].
We excluded the alleles bearing stop codons and the Group 1 sequences because they were considered non-functional.
However some of these alleles belonged to a pseudogene, whereas others were considered non-functional (Group 1 alleles).
For decades, pseudogenes have been considered non-functional or 'junk' DNA; however, the conserved sequence similarity between pseudogenes and coding genes suggests a selective maintenance of these non-coding elements.
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