On the other hand, milk samples with S/P indices ≤30 were considered negative, whereas samples with indices >30 were considered positive for C. burnetii antibodies.
Cases with no or weak staining were considered negative, whereas those with moderate to intense staining were considered as positive.
In the further analysis, the samples with 0 5% positive cells were considered negative, whereas samples with 5 50% positive cells were classified as weak HLA-DR positive, and those >50% as strong HLA-DR positive.
For CCR6 and CCL19, tumors with staining on less than 10% of tumor cells were considered negative while tumors with more than 10% of tumor cells stained were considered positive.
Titres < 16 were considered negative in accordance with the OIE Manual [ 6].
Thus, all titres of ≤ 1 10 were considered negative in accordance with OIE standards.
Tumors were considered negative, i.e. with loss of PTEN expression, when absence or reduction of immunostaining was seen in more than 50% of cells as compared with internal controls (i.e. vascular endothelial cells and nerves).
A sample was considered negative if amplification was observed with VIC but not with FAM.
Sera with CUs less than 20 were considered negative for DFS70/LEDGFp75 antibodies while those with CUs 20 100, 101 300, and >300 were considered low positive, moderately positive, and high positive, respectively.
Cases with a HER2/CEP17 ratio <1.8 were considered negative for HER2 amplification and those with a HER2/CEP17 ratio >2.2 were considered positive for HER2 amplification.
The culture will be considered negative after 48 hours of incubation with the absence of β-hemolytic colonies.
