Therefore, to consider inhibited digestive processes hindered by the unique structural characteristics, the following two extremes were used: Proteins without digestion represent a situation when all the cleavage sites were buried, while proteins digested with pepsin, trypsin and chymotrypsin simulate the case when all cleavage sites were exposed.
Next, quantitative kinase binding data is dichotomized as inhibited or not inhibited for each compound based on user-defined threshold (default: a kinase is considered as inhibited by the compound if IC50/Kd< 1 μM or percent of inhibition > 85%).
A p-value less than 0.01 and a z-score greater than 2 (prediction state: "activated") or smaller than -2 (prediction state: "inhibited") were considered significant.
Non-mitochondrial respiration rates were considered insignificant as myxothiazol-inhibited respiration was not detectable.
In addition, two of the eight (25%) isolates had no substitution in GyrA, but were considered positive for reserpine-inhibited efflux, while three isolates had a Ser-79-Phe amino acid substitution in ParC.
The highest dilution of serum that inhibited hemagglutination was considered the HAI titer.
After 24 h of incubation at 37°C, the MBC was considered the lowest concentration that inhibited visible bacterial growth on the agar.
The lowest concentration at which the plant extract inhibited bacterial growth was considered as the MIC value for the crude extracts.
During these procedures, behavioral observations (e.g., spontaneous talk, smiling, looking at the experimenter, and response latency) are obtained that are considered to be indicative for the inhibited temperament.
Presently, standard treatment includes administration of an antimuscarinic agent (e.g. atropine) and a reactivator of inhibited AChE (oxime), but is considered to be rather ineffective with certain nerve agents due to low oxime effectiveness of the currently available oximes, obidoxime and pralidoxime.
