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Reads which had length less than 25 bp after trimming were not considered for alignment.
Both the forward and reverse complement of each reference transcript were considered for alignment since the reference transcriptome was assembled from non-directional sequence data.
Therefore, the fraction of database sequences that have to be considered for alignment to the query, which Kim et al. call the 'filtration ratio' grows closer to one and the relative performance of miBLAST deteriorates.
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A BHIC is a cluster of conservation between species in which the alignment contains information for every species considered for the alignment.
Several different routes were considered for the alignment.
In case that a particular enterococcal species contained more than one protein from the same group of orthologs, only the protein with the lower e-value was considered for the alignment.
The partial alignment score of each matrix entry M [ i, j, (α i, β j )] is computed as the maximum of 6 types of values: (a) 0 (similarly to the classic Smith-Waterman algorithm, only non-negative scores are considered for local alignments).
We determined that >95% of the genes present in these other contigs are repetitive genes and sub-telomeric genes that were already present on one of the main chromosomes, so only the 11 large chromosomes were considered for the read alignment, as the other contigs would only increase ambiguity in the alignment without providing any extra information.
Homology was considered for sequences with alignment lengths >150 bp and sequence identities > 60% (with respect to the alignment length).
Lower registration rate on video collections actually reveals the fact that by using the photo collection codebook, only relevant video contents are considered for our model alignment problem.
Thus, using the seed and extend technique, only sequences sharing common kmers are considered for a possible alignment.
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