Note that the plants from individual single seedling plates were not considered as independent replicates, but instead the number of wilted plants per seedling plate was used as a disease index.
For arrays considered as partially exploitable based on several quality criteria additional hybridizations were done and considered as technical replicates.
The 12 values were considered as dependent replicates.
Thus, the microarray profiles of the same tissue type should not be considered as biological replicates.
Thus, three plants from each accession at given condition were considered as biological replicates.
Male and female reads from the sequenced tissues were pooled and considered as biological replicates.
In the Batman analysis, the tissue samples, distinguished in HCC and cancer-free hepatic tissues, were considered as biological replicates.
As parental strains, S and Strong-R of these two crosses were highly homozygous in responding to the resistance trait, these two crosses were considered as a replicates.
In addition, three samples were run on several microarrays, and were considered as technical replicates: samples from CBA/J, C57BL/6, and BALB/c mice were run on 5, 2, and 2 microarrays, respectively.
After obtaining a stable population of more than 100 daphnids in each beaker, a batch of juveniles was randomly removed once a week and put into fresh beakers (beaker-set B), which were treated in the same way as the corresponding beaker-set A and considered as biological replicates.
