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Therefore, the method can be considered alignment independent, and the use of the signal peptide feature is applicable even where sequence alignment deteriorates considerably.
For the paralinear distance analyses, the proportion of invariable sites in the considered alignment was estimated using likelihood and assuming a HKY85 + gamma + proportion of invariable sites model of DNA evolution.
So far, we have considered alignment of 2D images to references, but there is also a need to detect known features (motifs) in noisy and distorted image data in both 2D and 3D.
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This final MSA may be considered as some sort of average of all the considered alignments.
We only considered alignments of the sequences available in all five sensu stricto species, i.e., we excluded regions containing gaps in the alignment.
We have considered alignments based on class probability vectors where the original descriptors came from protein sequence (Bernoulli distributions) or structure (Gaussian distributions).
Provided fast methods are used to generate the original alignment, the meta-alignment procedure of M-Coffee can use a sparse DP procedure that takes advantage of the strong agreement between the considered alignments.
We only considered alignments to regions of high quality sequence and that had >95% identity to the query, ignoring cases where the Ensembl model itself was from a region of poor sequence quality (see Methods for details).
After that, the final tool path is modified by considering alignment error, tool radius error and squareness error.
These putative proteins share approximately 25-50% sequence identity to human GPCR pathway proteins when considering alignment lengths >100 aa.
A suggested way to solve this problem is to consider alignment as a random variable, a strategy developed into the statistical alignment procedure [ 49, 50].
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