Clustering on columns discriminates the treatments based on global considerations of the gene expression patterns.
Clearly, more research is necessary to confirm their consistent upregulation in H. desmarestianus spleen across additional variables (e.g., time points, body conditions, parasite load, etc).. Of additional interest is the consideration of these expression results in addition to the results of our selection tests.
In the present study, we included several considerations to detect the expression of OCT4A isoform in the mouse ADSCs, including treatment of all RNA samples with RNase-free DNase I, RT-PCR analysis using a forward primer specific to OCT4A, and immunostaining using a monoclonal antibody which specifically recognizes OCT4A protein [ 11, 12].
Following these considerations, the expression of the complete dkr ORF was investigated with all presented LAB systems/variants as well (see results in Additional file 1, Additional file 2, Additional file 3, Additional file 4).
A further consideration is the expression of the FcγR variant alleles in control and disease populations, which might also influence response to therapeutic mAbs [ 23, 24].
Conversely, miRNA families were excluded from consideration if the expression of any single family member was disconcordant with the expression profile of other family members within or across the three different arrays.
The expression value near of 1 is based on the biologically meaningful consideration that the expression level of 1, which means no difference between neoplastic and non-neoplastic tissue, separates the two groups into a high and a low expression group.
The possibility of opposite results by the two methods could be due to the reasons that genome analyzer provides a holistic picture of all the isoforms of a gene into consideration, whereas the expression by RT-PCR is specific to the isoform of the gene into consideration owing to the use of gene specific primers.
With these considerations, we compared the expression levels of Ki-67 and CT protein in ectopic thyroid tissues with orthotopic thyroid gland to evaluate the risk for malignant transformation.
We should also take into consideration whether there is difference of the expression of miRNAs among normal samples because of the contamination of intestinal metaplasia or dysplasia.
