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However, the ability to isolate cells occurring at such low frequencies within a population requires consideration and optimization of screening parameters.
In order to design and analyze experiments using real-time PCR, factors such as sample preparation, quality of the standard, choice of a housekeeping gene, and normalization of samples need careful consideration and optimization.
After careful consideration and optimization of this concept, a library consisting of 768 oligonucleotides was produced that can generate over 131,000 sequence optimized 11-mers.
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In this study, considerations and optimization data for each of these variables are presented.
Two generalized parallel-series ultracapacitor shift circuits are presented and analyzed, and some design considerations and optimization methods are discussed.
Design considerations and optimizations of Negative Capacitance FET (NCFET) are explored using simulations.
Design considerations and optimizations to achieve the low SS and hysteresis-free transfer were elaborated.
We introduce scaling considerations and process optimization along with experiments and SONOS device characterization.
We describe scaling considerations and process optimization to achieve low-voltage operation (+7 V write for 2.5 ms/−7 V erase for 7.5 ms) with 10-year retention at 150 °C.
This experimental work has been guided by the modeling work presented here as the theoretical considerations permitted evaluation and optimization of the synthesis conditions applied experimentally.
An important consideration for practical realization and optimization is the additional impedance matching condition imposed on the metal and dielectric building blocks [40, 75].
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