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This problem is normally encountered if the two alleles' distiguishing segments have considerably lower coverage than the rest of their sequence.
While a larger number of arrays may be able to compensate and provide increased statistical power to a glass long oligonucleotide array experiment, it is not relevant to the remaining issue of considerably lower coverage.
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Compared with reported coverage >95%, our 5 coverage assessments all showed substantially lower coverage.
For subsequent analysis we only considered cytosines that are covered by at least four reads (except for the ECM methylome, which has a considerably lower overall coverage).
Additional pilot sequencing of the 4d PF time point, which had been previously prepared in a similar fashion and sequenced on an Illumina Genome Analyzer at University of British Columbia Sequencing center serves as replicate for comparison, though with considerably lower sequencing coverage.
Not vaccinating children after 12 months of age provides only a 3-month-window for children to receive routine MV and based on our data it will lead to a considerably lower MV coverage.
However, general practice surgeries in Hackney known to have high proportions of Orthodox Jewish patients have considerably lower vaccination coverage (55%75%% of patients 24 months of age had received measles, mumps, rubella [MMR] vaccine in the 3rd quarter of 2012) compared with the London average (87.3%) (7 ).
With these parameters, effective coverage is generally considerably lower than population coverage, and exceeds 50% only when population coverage is close to 100% (Fig. 3).
These observations suggest that the coverage of protein-coding regions is considerably lower than the coverage estimated for the entire genome (~75%).
However, coverage of 5'UTR sequences (2.0-fold) and 3'UTR sequences (1.6-fold) was considerably lower than the coverage of ORF sequences (12.5-fold).
In a de novo assembly of three individual human genomes, best false negative allele calls were obtained with considerably lower (20) read coverage [ 29].
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