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We also found that sbrE (rli47), which has no homologies to ncRNA entries in Rfam, appears to be directly regulated by σB, based on the considerably higher transcript levels (186 fold) present in the parent strain as compared to the sigB-null mutant, consistent with results from a recent tiling microarray study [ 20].
Generally, Iogen-M8 shows considerably higher transcript levels of all tested genes under all conditions compared to Rut-C30 (compare Figure 2A-E to Figure 2F-J); e.g. transcript levels of xyn1 and xyn2 are 100- to 10,000-fold increased compared to those in Rut-C30 (compare Figure 2A to Figure 2F and Figure 2B to Figure 2G, respectively).
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Among the differentially expressed transcripts, considerably higher numbers of ESTs were represented as either hypothetical (unknown) proteins or no GenBank match suggesting those may be novel genes associated with internal defense of abalone.
qRT-PCR showed an absence of transcript over the deleted region, but the remaining exons appeared to give rise to a transcript of considerably higher abundance than wild-type Spen message.
As expected from the overall transcriptome aligned fraction, the fraction of An. albimanus transcript sequences that aligned to An. darlingi (transcript coverage) was considerably higher than for the An.
In addition, we found the relative abundance of antisense transcripts is considerably higher for differentially expressed genes than those genes whose expression levels remain unchanged (p = 1.35E-7, Wilcox rank sum test; Figure 2B).
In contrast, the expression of GLI1, GLI2 and SMO transcripts is considerably higher in fibroblasts than in epithelial cells [ 73].
Similarly, LT-SMN2 transcripts display considerably higher expression levels in CNS tissues with a >3-fold higher expression in spinal cord compared with muscle, heart and liver, suggesting that the amounts of SMN protein derived from LT-SMN2 are sufficient to modulate motor neuron degradation (Supplementary Material, Fig. S1).
We found that the BCL9 transcript levels were comparable in both cell lines to those in Wnt-stimulated HEK 293 cells, whereas the B9L transcript levels were considerably higher in the colorectal cell lines (35× and 50× higher in SW480 and HCT116 cells, respectively, compared to unstimulated HEK 293 cells; Fig. 3D).
The two probes hybridised to a single transcript of a size considerably higher than 9.4 kb (Fig. 1C).
The number of transcripts per cell was considerably higher based on oligoarrays and TransCount than based on MPSS and SAGE, both at high and low concentrations.
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