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Isolates belonging to genotype I share considerably higher sequence identity across the p72 gene compared to isolates from the sylvatic cycle, which suggests that this genotype probably evolved from a single source introduction (3 – 5 ).
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The rarefaction curve computed for the Titanium dataset was also far from reaching the plateau phase, indicating that considerably higher sequencing effort would be required to cover all phylogenetic groups of the underlying microbial community.
It possessed a unique N-terminal amino acid sequence of AIGPVADLKV, which has considerably high sequence similarity with that of other fungal laccases, but is different from that of C. comatus laccases reported.
The present laccase possesses considerably high sequence similarity with those C. comatus laccases or laccase-like proteins reported, but they are obviously different with the highest similarity of 80%.
Further confounding the problem is that the error rate per nucleotide for a given single read can be considerably higher than Sanger sequence traces (Mardis 2008; Quinlan et al. 2008).
A concatenated analysis of SSU rDNA sequences with Hsp90 sequences demonstrated considerably higher statistical support values for almost all of the deep nodes when compared to trees inferred from SSU rDNA alone [53].
However, there are cogent reasons for using a peptide-focused vaccine since the inductive power of the repositioned peptide is generally considerably higher than from full-length sequence [ 37].
In comparison, the success rate of intron-primed exon-crossing (based on salmonid intron sequences) was considerably higher: a single clear PCR product was obtained for 21 of 22 loci (95%) and high-quality sequences were obtained for 17 of these (77%) – i.e., a success rate almost four times higher than that obtained using the EPIC approach (χ2 = 7.771, d.f. = 1, P = 0.005).
In practice we found a group of sequences with comparable (very) low e-values (sequences with considerably higher e-values (separation < e−15).
Therefore, the metagenomics read assignment accuracy will be considerably higher when the proportion of sequences from known species is high.
Sequence identity was considerably higher in the protein core (58%) than the solvent exposed regions (25%), reflecting the selective pressure to maintain the same tertiary fold (Figure 2.1c).
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