Infrared reflection-absorption spectroscopy did not indicate considerable changes in secondary structure compared to FT-IR spectra in solution.
With ITS2 considerable sequence and secondary structure divergence existed among the eight species, but a combined sequence and secondary structure phylogenetic analysis confined to helix II of ITS2 corroborated relationships as inferred from the rbcL gene phylogeny.
From Figure 6, the two negative bands intensities of HSA at 208 and 220 nm increase with negative Cotton effect by the adding of CIT, which indicate the considerable changes in the protein secondary structure.
We interpret this as an indication of either their recent origin and rapid spread through the genome or considerable pressure for maintaining a hairpin secondary structure and note that this pattern is in contrast with other structured RNAs, such as group I and group II introns [ 52, 53], in which the loops tend to be more conserved than the helical regions [ 12].
Helix-mediated PPIs[ 9] have received considerable attention[ 10] as the secondary structure motif represents a generic pharmacophore.
In the past three decades, considerable success has been made in secondary structure prediction, e.g., with average accuracy of about 70% [ 11- 13], and offered a viable venue toward RNA tertiary structure prediction [ 4, 5, 13, 14].
In addition, the analysis indicates that despite considerable variation in size, sequence and secondary structure characteristics commonly found within a given category of transcripts (e.g. C/D box snoRNAs) their affinity for the core proteins component of the ncRNPs is not systematically affected.
These advances considerably extend the reach of sequence-based homology detection because secondary structure is conserved over considerable evolutionary distances, just as tertiary structure, and can be reasonably well predicted from sequence.
There exists a facile computational link between RNA sequence and secondary structure due to the considerable efforts toward RNA secondary structure prediction.
Considerable amounts of missing information on the existing fields like secondary structure, interacting proteins and experimental evidences in support of the PPI, have been added.
