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Since this condition was ideal for predictions of the motion trajectory, we expected considerable localization errors in motion direction.
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This complex (C4) showed high intensity fluorescence (quantum yield = 41.21%, τ = 14.57 μs) and considerable ER localization, but the localization was less specific compared to C2, with fluorescence exhibited in the nucleus and to some extent in the cytoplasm (Fig. 1).
Wild-type PIN1 and PIN7 overlapped with the FM4-64 signat athehe PM, whereas the 3 m1- and M3-mutant forms of PIN1 and PIN7 showed considerable internal localization, though they were partly localized in the PM as well.
If the mismatch between the actual and modeled bias becomes considerable, the localization error of the proposed algorithm can become higher.
Immunofluorescence staining showed that in sensitive Calu-6 cells, treatment with 3 µM AZD6244 induced considerable subcellular localization of FOXO3a from the cytoplasm to the nucleus.
Untreated primary MEF exhibit cell surface expression of both 5T4 and CXCR4 with considerable co-localization (Figure 7).
However, close inspection showed that while there was considerable co-localization of MSP119 and CRT at this stage, coincidence was not complete.
Merging the images demonstrated considerable co-localization of the proteins, although this was not absolute – single color puncta were also clearly apparent in most cells.
Using sequential IFA, we observe a considerable co-localization of H4K5Ac with Nup100 at the periphery of nuclei in the rings (Figure 6) and trophozoites (data not shown).
In addition considerable co-localization between the GFP protein and the cytoplasmic marker Pfactin, along with ER tracker (invitrogen) was also observed.
However, the co-expression with LRP6 elicited a marked redistribution of LRRK2, with considerable co-localization between the two proteins (Fig. 4A).
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