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The time course of pET22b-lacB biomass shown in Fig. 2c indicates considerable cell death at 24 h, whereas no cell lysis occurred in case of pET28a-lacB and pET32a-lacB.
The engineered polyplexes exhibited favourable physicochemical characteristics for transfection and dramatically elevated the level of Bid/tBid expression in both MUC1 over-expressing caspase 3-deficient (MCF7 cells) and caspase 3-positive (T47D and SKBR3) tumour cell lines and, concomitantly, induced considerable cell death.
In wild-type animals, considerable cell death in retina took place one week after birth, then the cell death became occasional at later stages [25].
stage, possibly due to the considerable cell death.
In all cases, there was considerable cell death in the lesioned regions, accompanied by striatal shrinkage.
At the highest concentration, ZnO nanoparticles are cytotoxic and lead to considerable cell death.
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In addition, microscopic observation of the cell cultures showed that a 72 h exposure to the higher BioEG concentration (0.2 g l-1) led to a considerable amount of cell death, since only few viable cells were present in the sample after incubation under such conditions.
With conventional assays, there is considerable amount of cell death even before pharmacological/molecular interventions are applied, masking the effect by interventions even if they induce further cell damage.
Regarding S1P, high concentrations of this lipid (10 µM) induced a considerable amount of cell death, which is in stark contrast with a previous report showing that 10 20 µM S1P prevents hESC apoptosis [28].
Treatment of B103 cells with exogenous Aβ also resulted in a considerable proportion of cell death (40% by the morphological assay and 38% by the MTT assay), which was inhibited by co-transfomation with pcDNA-HA-NIa but not by pcDNA-HA-mNIa co-expression (Fig. 5A and B).
Only Ad5hTRAIL infected cells exhibited considerable amount of cell death following transfection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com