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In addition, microscopic observation of the cell cultures showed that a 72 h exposure to the higher BioEG concentration (0.2 g l-1) led to a considerable amount of cell death, since only few viable cells were present in the sample after incubation under such conditions.
Regarding S1P, high concentrations of this lipid (10 µM) induced a considerable amount of cell death, which is in stark contrast with a previous report showing that 10 20 µM S1P prevents hESC apoptosis [28].
With conventional assays, there is considerable amount of cell death even before pharmacological/molecular interventions are applied, masking the effect by interventions even if they induce further cell damage.
However, later in culture they induced a considerable amount of cell death.
Only Ad5hTRAIL infected cells exhibited considerable amount of cell death following transfection.
Cultures in early plateau phase (where a considerable amount of cell proliferation is balanced by cell loss) showed a reduced sensitivity to bleomycin when compared with cells in exponential growth.
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Immunohistochemistry (IHC) also revealed that the T+I− group retained a considerable amount of cells expressing Fibronectin.
However at later time, when population starts approaching Hayflick limit (e.g. at t = 150), in SITS (but not in TS) a considerable amount of cells with short telomeres (i.e. stressed cells that accumulated many mutations) has been replaced by cells with longer telomeres and few mutations.
Interfascicular fibers accumulated a considerable amount of secondary cell wall material, although the autofluorescence signal was still weak compared to the vascular bundle regions (Fig. 1d).
Interestingly, many T192 cells exhibit a single TO (43.6%, Fig. 6) indicating that a considerable amount of daughter cells receive a single TO during the cell division process.
Delivery of sufficient viable cells can be quite restrictive for food manufacturers as a considerable amount of living cells are inactivated during food processing (heat, mechanical and osmotic stress), storage (exposure to acute toxic factors such as oxygen, hydrogen peroxide and water vapour) or during interaction with the matrix (Jankovic, Sybesma, Phothirath, Ananta, & Mercenier, 2010).
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