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Below we consider differentially expressed genes in W. smithii that, by virtue of their annotated function in D. melanogaster, are involved in post-diapause morphogeneis, or in the transition from potentially oxygen-poor winter diapause environments to oxygen-rich spring environments conducive to active development.
Even when we lower our threshold for what we consider differentially expressed genes (P < 0.05) we find a very high level of agreement (86%).
When we look at just the direction of expression, or the fold-change in expression across the genome, we find results very similar to those obtained when we only consider differentially expressed genes.
In general, this work exemplifies how strategies that not only consider differentially regulated genes but also regulatory elements of the stress response provide a more comprehensive view of bacterial gene regulation.
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A gene was considered differentially expressed if the moderated t-test resulted in a corrected p-value <0.01.
We conclude that disorders and drugs affecting the indoleamines must be considered differentially in the auditory and the visual system.
Signals were considered differentially detected if p<0.01.
Genes were considered differentially expressed when p-values were <0.05.
The techniques disagreed for 1,686 (3.8%) probesets, 809 of which were considered differentially expressed by Messina alone, and 877 of which were only considered differentially expressed by limma.
Only proteins with spot volumes consistently different in all replicate gels were considered differentially expressed.
In the case of tumor groups, genes that obtained a p-value<0.05 were considered differentially expressed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com