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The 16S rRNA gene was amplified by PCR using forward primer F-d1 5′-AGAGTTTGATCCTGGCTCAG-3′, and reverse primer R-d1 5′-AAGGAGGTGATCCAAGCC-3′, designed from base positions 8 27 and 1541 1525, respectively, which were the conserved zones within the rRNA operon of Escherichia coli (Gurtler and Stanisich 1996).
Ribosomal gene secondary structures have conserved zones that make it easier to reconstruct the structures of unknown homologous RNA sequences by direct comparison.
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This study also searched the highly conserved zone of polarizing activity regulatory sequence (ZRS) for other SNPs that might explain this trait and found none.
Therefore, lacZ3 oligonucleotide primers (Table 1) were designed to amplify a highly conserved zone of lacZ, resulting in a 234 bp PCR product (Fig. 1b).
You might have imagined that a marine conservation zone would be a zone in which marine life was, well, conserved.
Our findings suggest that the germinative zone is conserved in zebrafish.
Key targets include conserving in protected zones at least 17% of the world's terrestrial and inland water areas and 10% of coastal and marine areas, halving the rate of loss of all natural habitats, including forests, and preventing the extinction of known threatened species.
However, zones of relatively conserved sequences (e-values<1e-80) are also evident in comparison with other genera (Fig. 2a), and a concatenated BLAST Heat Map, created using custom databases of Clostridium clusters, displays flares with members outside of Clostridium cluster IV (Fig. 2b).
It is possible that thylakoid tip convergence zones are a conserved feature of algal chloroplasts, as similar structures can be found in a wide range of algae (Gibbs, 1962; Moisan et al., 2006).
We provide an in-depth analysis of each of these subsets, and show that while conserved subsets provide the safe zone for which yeast can be used as an ideal model organism, the human-specific subset can shed light on the shadowed subspace of the human interactome in yeast.
They involve dividing the cardiovascular system into a number of compliant zones or compartments and as the blood passes through each zone it must be conserved.
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