In addition, alanine mutations were placed at the processing sites flanking the N and C termini of protease to prevent processing at these sites by the embedded protease [13], while the protease sequence itself was conserved as wild type (Table 1).
In general terms such marked differences were not observed in glucan levels however, phr1−/−, pga62−/−, pga4−/−, dfg5−/− and ssr1−/− have significantly increased cell wall glucan content, while the mutants mid1−/− and pga31−/− conserve their wild type glucan levels (Fig. 5).
Similar data were observed for ROCK2, showing that the forced expression of miR-200b/c in TFK-1 cells results in a decrease in luciferase activity using conserved wild-type binding sites compared with a mutant sequence and also indicating that the conserved predicted binding sites of miR-200b/c in the 3′-UTR of ROCK2 were functional.
One-band fragment conserved in the wild type and lost in the mutant bacteria was found to code for the lipase gene.
Given that G/C tracts are unstable in the C. elegans dog-1 mutant, we wanted to test if G/C tracts were conserved in other wild type isolates of C. elegans or whether G/C tracts are inherently unstable.
Hence, these TFs appear to be conserved in both rad mutant and wild type and are most likely involved as transcriptional activators (Additional file 2).
The two fragments found in the wild type digest were conserved in the mutant digest suggesting that neither was disturbed by the insertion.
But 102 phage restriction value reflects the ability of the new RMS to protect cells against foreign DNA invasion; so this function of the wild type RMS was conserved in the RM.Eco29kI RMS.
These non-human mammals generally showed the conserved nucleotides present in the human wild type, not the mutations to Runx2 concentrated in the DNA binding domain among human CCD patients (see Tables 1 and 2 in [ 19]), except for a sequence of Canis.
The rs1935881 wild type allele A is conserved in several mammals, while the G allele of rs1342913 is conserved back to zebrafish (Figure 1).
