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CNE1 spans a 945 bp human/fish conserved track with overall human/fish sequence similarity of ∼71%.
Starting from the "most conserved" track for each alignment generated by the Phastcons program, we padded the genomic coordinates by 25 bps on both sides.
The UCSC browser TFBS conserved track was implemented with a Z score cutoff of 1.64 [ 19].
Transcriptional profiles and protein-protein interactions were taken from the Transcription Factor Binding Site (TFBS) conserved track in the UCSC genome browser [ 10] and HPRD [ 11] databases, respectively.
For TFs, MAGIA2 utilizes experimentally validated TF– miRNA interactions from mirGen2.0 and TransmiR and on TF gene interactions from the 'TFBS conserved' track of the UCSC human genome annotation.
To perform this analysis, we used UCSC's [30] "Most Conserved" track and phastCons conservation score to filter our candidate COUP-TF binding site list.
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Furthermore, from the transcription factor binding site conserved tracks in UCSC, we find this CE1 homologue site is a potential transcription factor binding site of Chx10 conserved in both human and mouse [ 24].
CNE6 docks a small moderately conserved human/fish track of 179bp, within human/mouse 862 bp track with overall 87% conservation.
Potential functional SNPs [those that were located in coding regions or were evolutionarily conserved based on the conservation track of the UCSC Genome Browser University of Californiaa, Santa Cruz; http://genome.ucsc.edu) were also given priority.
It is located in a genomic region highly conserved between several vertebrates according to the conservation track of the UCSC genome browser.
Missense mutations were highlighted if they occurred within highly conserved residues in vertebrates, assessed utilizing the UCSC Genome Browser (http://genome.ucsc.edu/) conservation track tool [ 17].
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