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This strategy is based on the assumption that the organelles would be sufficiently conserved to allow specific recognition of heterologous Bax protein.
All twelve BAC probes derived from M. sexta cross-hybridized on the corresponding A. convolvuli chromosomes (Fig. 3), indicating that the underlying sequences are well-enough conserved to allow the generation of specific signals, despite the species differences.
The COI gene may be too conserved to allow resolution of divergence caused by the most recent glaciation.
With the very restricted phylogenetic differences found within each subtype, the chosen gene product was too conserved to allow for analysis of transmission chains.
It also raises questions for those engaged in vaccine development about whether features found on the protein surface are sufficiently conserved to allow the immunogen-mediated induction of a broadly neutralizing immune response.
The first two related criteria are the most obvious ones: i) a gene has to be (nearly) universal, i.e., represented by readily recognizable orthologs (preferably single-copy) in all cellular life forms; ii) the sequence of the gene in question has to be sufficiently conserved to allow the construction of an unambiguous alignment and an informative tree.
Similar(53)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism to allow cells to adapt to stress targeting the endoplasmic reticulum (ER).
When excluding the smallest database, Macrostomum lignano, for which only 1,231 cDNA fragments were available, we obtained 43 genes that were unambiguously present in all remaining taxa and conserved enough to allow alignment.
In spite of this variability, C-terminal domains are conserved enough to allow reliable alignment.
However, the 8 CM C-terminal domains are conserved enough to allow multiple alignment construction and phylogenetic analyses.
Although CenpC homologs are not as easily identifiable as CenH3, two motifs are conserved enough to allow cross-species homology searches.
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