We analysed the expression of SVSP genes in a set of phenotypically different cell lines, established by infection with cloned T. parva parasites, to investigate whether the pattern of expression is conserved or changes depending on parasite genotype, or host cell type and background.
We attributed "0" (zero) value to the absence of a character (an association or a syntenic group that is conserved or changed by chromosome rearrangements) and "1" (one) value to the presence of the character.
Response regulator aspartate phosphatases as well as their cognate aspartate phosphatases are either not conserved or exhibit changes that make them difficult to recognize by sequence similarity.
Most of the amino acids that form the binding pocket of the FHA domain, those that comprise the FHA/BRCT1 interface, and those that contact phosphorylated residues are precisely conserved or have conservative changes.
We conducted an evolutionary analysis to determine if miRNAs found in R. microplus were perfectly conserved or accumulated nucleotide changes since the last common available ancestor (See Methods).
Ultimately, this will also reveal the extent to which the conserved or divergent expression changes are subject to adaptive and non-adaptive forces during evolution.
We conducted an evolutionary analysis to determine if miRNAs found in R. microplus were perfectly conserved or accumulated nucleotide changes since the last common ancestor based on available sequences for each miRNA (miRBase release 17).
Nine (50%) of the sequence changes affecting conserved or shared predicted ESEs are currently designated as UVs.
If those sites are particularly variable or particularly conserved, the change in their location relative to the bin center can substantially affect the quality of the model fit.
Focusing on the "well behaved" or conserved gene changes alone would not only impede the choice of sample collection, but would also make conclusions and findings more subjective by selecting only those parameters that define the subsets of a population containing these conserved changes.
Conserved site changes or polymorphic site changes observed in >1 patient were confirmed by phenotyping, as previously described.
