Sentence examples for conserved internal control from inspiring English sources
Two highly conserved internal control regions, the A and B boxes, together form a specific binding site for the multisubunit transcription factor IIIC (TFIIIC).
tDNAs in the fission yeast genome range in size from 71 102 bp and are characterized by highly conserved internal control elements.
Primers for the internal control (ACTIN) and conserved primers that amplify all GPC paralogous and homeologous genes in a region outside the RNAi construct have been described previously [ 3].
At somite stages it conserves the contro-lateral side as an internal control.
Based on the published sequences, conserved sequences of 10 target genes and the internal control of β-actin were employed to design degenerate primers of each gene.
The highly conserved U6 small nuclear RNA (snRNA) was taken as internal control (forward: 5′-CGCTTCGGCAGCACATATACTAA-3′ and reverse: 5′-TATGGAACGCTTCACGAATTTGC-3′) [ 16].
The assay was carried out using two sets of primers designed to amplify highly conserved sequences of the nucleocapsid gene of BCoV and the internal control, bovine glyceraldehyde-3-phosphate dehydrogenase, RNA.
A unique feature of most Pol III promoters is the presence of internal control regions (ICRs) that are composed of conserved sequences separated by more variable regions.
Reactions were performed on 100 ng of total DNA from biopsies, amplifying simultaneously both the highly conserved bacterial 16S rRNA gene and the eukaryotic RNaseP gene, used as an internal control for assessment.
The multiplex PCR was optimized for the amplification of a 340 bp fragment of the second internal transcribed spacer (ITS-2) of F. hepatica rDNA, while another set of primers was designed and used to amplify a conserved segment of the nuclear 18S rDNA of the snail (451 bp), as an internal control of the reaction.
PCR primers were designed using sequences that were conserved among the three species and primers for β-actin were used as an internal control for normalization (see Table 4).
