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The conservation within these secondary structure H-bonds is what leads to conserved flexibility along the backbone.
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What is the significance for maintaining the evolutionarily conserved conformation flexibility in the RNA-binding ring of the archaeal exosome and PNPase?
As with single protein (intra-EC) predictions, evolutionarily conserved conformational flexibility and oligomerization can result in more than one set of contacts that must be de-convoluted.
4. Conserve capital to maintain flexibility.
Are regions that convey conformational flexibility conserved over evolutionary time?
Preserve flexibility.
Maintain flexibility.
However, maintain flexibility.
With the H297A mutant, the hydrogen bond formed by His297 and Ser299 was no longer conserved, thus increasing the flexibility of the loop between 293-303, weakenedakened the interaction between Tyr300 and GlcNAc.
Furthermore, this flexibility is conserved across Notostraca, and may have been maintained for at least 250 million years.
Compared to the large differences within the thermodynamic predictions, predictions of backbone flexibility are more conserved.
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