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Cells display highly conserved features, such as the (almost) universal rule of the genetic code, as well as the DNA replication machineries.
Numerous studies have demonstrated that bactericidal activity is independent of highly conserved features, such as invariant disulfide array, Arg-Glu salt bridge or Gly residue at CysIII+8 I, with exception to the high arginine content relative to lysine (Lehrer, 2007; Rajabi et al., 2008; Schmidt et al., 2012), which complies with our finding.
Borderline cases were resolved by inspection of conserved features such as the presence of known catalytic residues.
Nevertheless, the conserved features such as DNA-binding-α-helix and Fe2+ and Zn2+ binding domains could be identified [ 71].
All the proteins have distinct conserved features such as a metalloproteinase-like domain, a disintegrin-like domain, a cysteine-rich domain, a prodomain, and a transmembrane domain (also known as ADAM family) [ 77].
Three well-known TSS prediction tools are incorporated with orthologous conserved features, such as CpG islands, nucleotide composition, over-represented hexamer nucleotides, and DNA stability, to construct the novel Gene Promoter Miner (GPMiner) using a support vector machine (SVM).
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This results suggests that we can safely combine simple sequence-based features with evolutionarily-conserved features such as domains without suffering any performance loss due to excessive features.
The GPMiner system uses a SVM that considers orthologously conserved regulatory features, such as CpG islands, nucleotide composition, over-represented hexamer nucleotides, and DNA stability, of a promoter sequence to identify mammalian proximal promoters.
We investigated approximately 200 kb of the genomic region surrounding the ETV6 locus in Fugu (fETV6) in order to find conserved functional features, such as genes or regulatory regions, that could give insight into the nature of the genes targeted by deletions in human cancer cells.
Nevertheless, Arl8 GTPases do conserve certain Arf/Arl features such as the asparagine of the N-[VI] motif and a tryptophan directly preceding the Walker B aspartate.
Conserved features important for functionality, such as a long N-terminal extracellular domain (ECD), seven transmembrane domains and a short C-terminal intracellular domain, were identified in both predicted proteins.
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