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Moreover, the target for this insertion was a GGCACCA motif, found both at the end of the tRNA, and identically conserved at the end of the insert.
The location of this active site sequence is highly conserved, at the end of the canonical β2 strand and into the following α-helix, so that the N-terminal Cys is in an extended conformation, while the remaining three amino acids assume α-helical secondary structure.
Uracil and adenine was highly conserved at the 5' end of 21nt and 24nt sequences, respectively.
Pseudo-uridines are conserved at the 5' end of U1 snRNA of yeast and human.
Also conserved at the 5' end of the TRA/D locus in opossum, human, and mouse are TRA V genes (TRAV) interspersed with the OR loci.
Similar(55)
The gene is similar to yeast Vps13 and is most highly conserved at the ends of the protein which are similar to vacuolar protein sorting domains [ 11].
10.7554/eLife.00078.006 Figure 4. Chromatin architecture is conserved at the 5′ end of transcripts across eukaryotes and archaea.
In LAVA at most five repeat units are conserved at the 5' and 3' ends of the domain, respectively.
Foundation trenches were found at the west, north, and east ends of the building, and there is another trench, partially conserved, at the south (Figure 7).
In general, the CRISPR repeats are not conserved at the sequence level beyond a few short conserved sequences such as GTTTg/c at the 5′-end and GAAAC at the 3′-end.
Secondly, the hydrogen bonding between the conserved asparate at the end of strand 4 and the nearly absolutely conserved threonine C-terminal to strand 5 help in stabilizing the curvature of the central sheet (Fig. 4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com