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In addition, alanine mutations were placed at the processing sites flanking the N and C termini of protease to prevent processing at these sites by the embedded protease [13], while the protease sequence itself was conserved as wild type (Table 1).
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The Bristol strain N2 is used as wild type.
For morphological comparison wild type littermates were used as wild type controls.
Aspergillus nidulans A4 was used as wild type reference strain.
w[1119] was used as wild type and is indicated by "+".
CS flies were used as wild type.
y w served as wild type.
Bristol N2 was used as Wild Type.
Oocytes carrying eb1-gfp and wild type for klp10A are referred to throughout as wild type.
One-band fragment conserved in the wild type and lost in the mutant bacteria was found to code for the lipase gene.
As a result, It was found that mutants I288A, G296A, Q347A, L350A, L468A and V495A had a recombination activity as wild-type integrase, showing that these conserved residues were not important for recombination.
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