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Furthermore, there is also evidence for selection against indels that do not conserve the reading frame as these are less likely to be shared among subspecies.
The distribution of coding indel size is shown in figure 5 B. There is an excess of deletions compared with insertions and a strong enrichment of indels that conserve the reading frame (around 65%).
For segregating variation, indels that disrupt the reading frame have an excess of rare variants compared with indels that conserve the reading frame (fig. 5 B) also suggestive of selection against the former.
However, the accumulation of viruses with large genomic deletions due to in trans expression of virus' genes will also depend on mutational supply, specifically how often recombination generates viruses with deletions in NIb that conserve the reading frame in downstream genes.
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We identified 64 different deletion variants, most of which conserved the reading frame.
These two deletions (nucleotide positions 7621 8484 and 7046 8500) conserved the reading frame and were situated within the NIb cistron (positions 6982 8517 of the wild-type genome).
Obtained sequences were translated to protein conserving the reading frame after the AD GAL4-activating domain and analyzed by BLASTP against a database of L. major predicted proteins.
Obtained gene sequences were translated to protein conserving the reading frame after the AD GAL4-activating domain (http://www.expasy.org/tools/dna.html) and analyzed by BLASTP (http://www.genedb.org/genedb/leish/blast.jsp) against a database of L. major predicted proteins (www.genedb.org).
All of the non-insertion-deletion mutations conserved the open reading frame for ARF, suggesting specific targeting of the p16 locus for inactivation, a phenomenon reported in other tumor types as well [34].
If NANOGP1 did indeed remain functional after its formation, we would expect natural selection to conserve the sequence within its reading frame when compared to NANOG.
The more eye-friendly black and white screen, also, can be used for extended reading, again helping to conserve the main battery.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com