Embedding was performed using plastic resin, which proved to be the only robust method to acquire 3 µm thin cross-sections while conserving the cellular structure.
Although the cysteines are not conserved and the cellular FATC domain is not likely to always exist in an oxidized state, the helical structure is likely conserved (Lempiäinen and Halazonetis 2009; Sturgill and Hall 2009).
The first is to conserve cellular resources, and the second is to provide a delay to evaluate the severity of the damage and, depending on its level, reprogram gene expression either towards apoptosis or to a pro-survival repair response.
In addition, the means by which Smo relays the status of pathway activation to the Gli proteins do not appear to be evolutionarily conserved [4], particularly the cellular microenvironment in which Smo is activated and the downstream components it interacts with.
This mechanism may help to conserve cellular resources by minimizing the amount of maintenance performed by topoisomerases/gyrases, a strategy that may provide a significant advantage in the harsh conditions that prompt sporulation (i.e., starvation).
Autophagy is a conserved cellular pathway that maintains cellular homeostasis via the degradation of cytosolic contents and proteins.
In addition, Zhang et al. [ 71] recognized that scaffolds seeded by human fetal mesenchymal stem cell (hfMSC) reached cellular confluence earlier with greater cellularity and also conserved high cellular viability in the core of them compared to a static culture.
Ferritin is degraded in the lysosome and recent evidence implicated autophagy, a conserved catabolic cellular pathway, in the 'ferritinophagy' process (Radisky and Kaplan, 1998; Asano et al., 2011).
The mild increase in temperature alone did not cause up-regulation of the genes considered to be part of the conserved cellular stress response (e.g. HSP40, HSP70 and peptidyl-prolyl-isomerase [ 61]).
