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10 mM NaN3 was used to conserve samples for determination of organic carbon.
To conserve samples we did not confirm positive results by RIBA but instead tested for virus in plasma by using a real-time reverse transcription (RT PCR assay for HCV RNA developed in our laboratory.
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To circumvent some of these issues, multiplex immunoassays have been developed, which include planar micro-spot based (e.g., multi-array from meso scale discovery) and bead-based detections (e.g., Bio-Plex® from Bio-Rad) to increase throughput (i.e., multiple analytes vs. single analyte) and conserve sample volume [ 23– 25] (Table 1).
After finding a new species, he is careful to engage in "germplasm banking": conserving samples by storing them in a local repository.
Ethanol conserved samples embedded in LR White low viscosity resin yielded the best results with regard to both signal intensity and resolution.
RNA Protect samples were closer to the controls than RNA Later conserved samples.
However, a larger variability was observed in RNA Protect conserved samples during storage as expected.
Within 9 months after collection, the methanol HCl conserved samples were transmethylated by heating at 90 °C for 4 h.
Accordingly, RNA Later conserved samples are more dissimilar to the controls than samples stored for less than 24 h in RNA Protect.
Alteromonadaceae and Microviridae transcripts were significantly more abundant in the controls than in the RNA Later or RNA Protect conserved samples.
RNA Later conserved samples had a reduced abundance of e.g. Prevotellaceae transcripts and were depleted for e.g. COG category "Carbohydrate transport and metabolism".
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