Two putative N-linked glycosylation sites are conserved at the same positions among all species other than cat.
The 5'-splice consensus site is also conserved at the same position as in human, mouse, rat, and other higher vertebrates.
The extracellular domain of Tetherin/BST-2 has two putative N-linked glycosylation sites, which are highly conserved at the same positions among human, rhesus monkey, dog, pig, rat, and mouse, and orthologs have been identified that are actually glycosylated heterogeneously [8], [9].
Among them, one intron position is conserved at the same phase in DmPsGEF, TcPsGEF, and PhcPsGEF.
Intriguingly, the position of one out of two introns is conserved at the same phase in five species.
This type of the alignment enables the tool to detect regulatory elements that are phylogenetically conserved at the same genomic positions in different species.
In addition, the pancrustacean gene order nad5,trnH,nad4,nad4L is conserved at the same location of the strand in all amphipod mitogenomes studied (and it appears in other non-pancrustacean invertebrates as well).
These results demonstrate that some but not all intron positions are conserved at the same phase in the C2, PDZ, and RhoGEF domain-coding regions of five PsGEFs from different species.
In Drosophilidae, the exon-intron structure of the OXPHOS genes is completely conserved in 61 out of 68 orthologous gene clusters; 117 out of 127 intron positions are conserved at the same nucleotide position throughout the species studied, and only 10 discordant positions in seven different genes were found.
Comparison of human, mouse, rat, cow, and dog orthologous TRIM and TRIM-like genes showed that >99% of splicing acceptor and donor sites were conserved at the same relative position in the coding sequence in all species, i.e. the gene structure of TRIM genes is identical among different mammals.
