As is the case in mammals, PGC1α appears to be conserved as a target of TH in zebrafish, and probably also in other ectotherms.
In contrast, S6K is a conserved as a target of TORC1 in the green lineage [ 71], suggesting that the TORC1-containing mTOR complex could be the prototypical pathway for TOR signalling.
Interestingly, ESR2 and AR kept very few targets in the absence of FOXL2, whereas NR5A1 conserved a similar number of targets.
The background set consisted of any expressed gene that had a conserved miRNA target site with a context+ score ≤0.2 to any broadly conserved miRNA (Friedman et al. 2009), excluding all potential targets to the miRNA being tested.
Using a variety of approaches, we provide evidence for direct PAI-1 regulation by miR-143 and -145 through a non-conserved 8-mer target site and a conserved 7-mer target site, respectively.
Similar to C17ORF49, this miRNA site is in a region of high conservation between tammar and human and accordingly may represent a conserved miRNA target site.
Vista alignment between human and tammar indicate this gene has a highly conserved exon structure between these two species, with a conserved miRNA target in the 3'UTR.
This provides genetic evidence that the ribosomal RNA is a conserved cellular target of 6AP in Drosophila.
For example, a conserved miRNA target was identified in the 3'UTR of the gene Lrtm1, although the usage of this particular miRNA target varies across species.
We observe a synergistic effect of 6AP with deletions of the ribosomal DNA locus, suggesting that the ribosomal RNA is a conserved cellular target of 6AP in Drosophila.
