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In Heath et al. [27], the pathway must conserve a minimum number of carbon atoms from start to target compound.
In Boyer and Viari [39], pathways must conserve a minimum number of atoms which do not necessarily need to be carbons.
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This filter left a pool of 125 potential ncRNA candidates that are conserved in a minimum of four of the six genomes.
The output from blastx analysis of predicted peptide sequences was filtered to search for homologous sequences using an e-value cutoff of 1e-20, a percent match of 40% (true matches, not conserved), and a minimum match length of 30 for the high-scoring segment pair.
Accordingly, we derived a total of 101 ICS epitopes for H7N9, containing multiple 9-mer frames predicted to bind to many HLA alleles and conserved in a minimum of three of the four human-derived avian-origin H7N9 sequences (Table 1).
"Conserve a Legacy," through April 30.
They encourage people to conserve a nonrenewable resource.
Thereafter, from the total identified conserved genomic blocks, a minimum of 95% of sequence identity was set to identify highly conserved regions (>50 bp).
Primer pairs MDH (m), GAPDH (m), UBQ (m) and EF1-α (m) each targeted conserved sequences in a minimum of two known transcripts within their gene families (Table 1).
We used the following settings for the 50 species dataset: minimum number for a conserved position: 26; minimum number for a flank position: 26; maximum number of non-conserved positions: 8; minimum length of a block: 10; allowed gap positions: with half.
For the large dataset non-conserved sites were excluded from likelihood analyses making use of the Gblocks software [ 52], with the following parameter settings: minimum number of sequences for a conserved position: 55; minimum number of sequences for a flanking position: 55; maximum number of contiguous nonconserved positions: 8; minimum length of a block: 10; allowed gap positions: with half.
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