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Summary of miRanda Output of Conserved miRNAs Predicted to Bind linc-MD1, Related to Extended Experimental Procedures Conservation was evaluated using the Mammal Cons phastCons30wayPlacental. (1) miRNAs not expressed in muscle (see Extended Experimental Procedures).
Linc-MD1 was displayed using the UCSC genome browser (Fujita et al., 2010 ); genomic locus conservation was evaluated using the Mammal Cons phastCons30wayPlacental (Siepel et al., 2005 ).
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The statistical significance of the differences between average evolutionary conservation levels was evaluated using the Students' t-test.
The cross species conservation of the nonsynonymous SNPs was evaluated using the UCSC genome browser.
Evolutionary conservation of the mutated amino acids was evaluated using ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/). Frequencies of coding and splice-site GCH1 variants in cases and controls were compared by means of Fisher's exact (statistical significance set at P-value < 0.05 using a two-tailed test) and odds ratios (OR) and 95% confidence intervals (CI) were calculated.
Locomotor recovery was evaluated using BBB score.
Depression was evaluated using the Center for Epidemiological Studies Depression Scale CES-D Scale CES-D
Binding was evaluated using flow cytometry.
Lipid peroxidation was evaluated using PAR immunohistochemistry.
Statistical significance was evaluated using ANOVA.
Cell rounding was evaluated using ImageJ program.
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