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Minimal sequence conservation, short gene lengths, and low complexity sequence can hinder bacteriocin identification, even during gene calling, so they are often discovered by proximity to accessory genes encoding maturation, immunity, and export functions.
Bootstrap values of some nodes of the tree were found to be moderately low; this finding in the global OsWRKY analysis was not completely unexpected due to the low degree of conservation, short length of the WRKY domain and to the large size of the OsWRKY gene family.
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The concept of surrogate species, those whose protection affords the protection of other species, is considered a conservation short-cut.
A few reserves have been badly managed by conservation charities short on cash; others have lost the species they were established to protect.
Conservation of short sequence sites can be assessed using different metrics.
The conservation of short peptide motifs is associated with transient transactions and conservation of larger domain motifs is associated with more stable protein-protein interactions [34], [47], [48].
Second, conservation of short degenerate binding sites for RNA-binding proteins may be revealed by cross-species sequence comparison as has been done for C. elegans [46].
While the shorter singleton HPs may have arisen by chance, it is likely that some of them are functional genes that are difficult to homologize with other closely related sequences, given the problems with assessing percent conservation across short sequences with even minimal differences.
High-throughput studies have utilized various algorithms to detect conservation of short ORFs.
Fourth, longer introns are more likely to carry a segment of conservation than short introns (Haddrill et al. 2005).
In addition, the unorthodox methods used (complete sequence conservation over short sequence fragments) are hard to evaluate given the lack of a history for such methods.
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