Sentence examples for conservation of the active from inspiring English sources

In the case of APAO, instead, there seems to be a lower evolutionary pressure towards strict conservation of the active site residues, the only residues conserved in more than 90% of the sequences analysed being Trp62, His64, Tyr430, Ser473 and Thr474 (ortholog to SMO residues Trp80, His82, Tyr482, Ser527 and Thr528), besides the catalytically important Lys315.

The remarkable conservation of the active site area explains the identity of function between eukarial and prokarial tyrosinases with eukarial enzyme acquiring during evolution specific molecular determinants such as the Cys1 domain, the TM domain and the seven N-linked glycans.

An alignment of all cathepsin L predicted protein sequences from T. castaneum and T. molitor demonstrated sequence conservation of the active site residues QCHN in the majority of the sequences (Additional file 2: Figure S1).

Nonetheless, Cg-Slx1-DNA interactions can be modeled based on the conservation of the active site between Slx1 and GIY-YIG restrictases, for which high-resolution protein-DNA structures are available.

Hence there is a need for CA isoform specific inhibitors to avoid off-target CA inhibition, but due to the high amino acid conservation of the active site and surrounding regions between each enzyme, this has proven difficult.

Crystallographic analyses have also revealed the PD-(D/E XK fold in proteins that do not function as deoxyribonucleases at all and exhibit no conservation of the active site with the above-mentioned enzymes.

Alignments yielding an E-value≤0.5 were selected and divided into four classes: Class A: alignments showing complete conservation of the active-site region, Class B: alignments showing partial conservation of the active-site region, Class C: alignments with proteases lacking an active-site annotation, and Class D: all other alignments.

However, the conservation of all the active sites in the conceptual translation of K. foliaceum tyrC (Figure S2) and its transcription both imply that the protein is functional.

Superposition of the DraIII closed structure to Hpy99I, PacI and T4 Endo VII shows the high structural conservation of the HNH active site (Fig. 4).

JMB built the homology model, analyzed spatial vs. sequential conservation of the putative active site, and wrote the manuscript.

Because of the remarkable conservation of the peroxiredoxin active site, the same antibody is able to detect rhythms in mice, flies, fungi, plants, archaea, and bacteria.