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Conservation of response elements between species has been previously observed.
Genes demonstrating hypoxia-inducibility at 1 dpf, and therefore conservation of response across several phases of development, were preferentially chosen for MO knockdown in a secondary functional acute hypoxia sensitivity screen (Table 1, Figure 3).
Similarly, the hydroxylase inhibitor dimethyloxalyglycine, the cytokine CCL3, the TLR activator PolyIC (Fortier et al., 2004) and the nerve growth factor NT3 were effectively translated and predictable at a level comparable with the observed conservation of response S (Fig. 3A) when based only on activation of protein phosphorylation.
Furthermore, analysis using BaP-modulated genes in Oikopleura and human macrophage cells [ 37] showed an enrichment of shared pathways such as DNA-damage response and apoptosis (Additional file 1, Figure S5 A and B), suggesting a conservation of response to genotoxic stress.
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To explore conservation of responses across species, gene expression from the rat liver studies were compared to in vitro data from rat and human primary hepatocytes exposed to the triazoles.
In order to assess conservation of responses to BaP in mammals and Oikopleura, we compared pathways significantly affected by BaP in this experiment and in a gene set from a microarray experiment using human macrophages [ 37].
Many oxidative stress and apoptosis related pathways were affected in both Oikopleura and human cells (Additional file 1, Figure S5 A and B) suggesting conservation of responses to genotoxic carcinogenic compounds.
A similar accumulation of amino acids has been observed recently in Lotus japonicus and Populus euphratica, indicating a conservation of this response to high salinity [38], [39].
There is no significant difference between conservation of TCS response regulators and that of one-component EBPs.
Calorically restricted mice have been shown to upregulate phase I xenobiotic-metabolizing enzymes (Steinbaugh et al., 2012), pointing at conservation of this response.
The conservation of the response of genes encoding hemicellulases and cellulases to carbon catabolite de-repression in several ascomycetes, suggests conservation of the role of carbon starvation in the induction of lignocellulose-degrading CAZymes during fungal exposure to lignocellulose.
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