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Conservation of fungi faces the challenge of high species diversity, limited knowledge and a general lack of public awareness.
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Trade, harvest, and conservation of caterpillar fungus (Ophiocordyceps sinensis) in the Himalayas.
Understanding of the genetic diversity and genesis of O. sinensis will provide important information for the evolution and conservation of this fungus.
Given the strong conservation of motifs between fungi and animals in the DNA-binding and dimerization domains, we hypothesize that the molecular function of these fungal proteins will be very similar to their animal homologs, i.e., they are transcription factors that will bind DNA targets, most likely as dimers (like GRH).
Because of the complexity of conidiophore and conidial development and their relatively poor evolutionary conservation among taxonomic classes of fungi, further characterization of candidate genes involved in asexual development in C. zeae-maydis will require functional characterization such as targeted disruption.
Correlated expression levels have also been found to increase the likelihood of conservation among fungi.
The high conservation of these factors in fungi underlines their important role.
FUNGIpath further allows the conservation of pathways between different fungi to be checked and visualized.
We have therefore conducted exhaustive BLAST searches of publicly available sequence data (see Methods) to asses the presence and conservation of CSL family members in fungi.
Notably, deletion of 5 homologous genes are sensitive to DNA damage reagents in S. cerevisiae (Table 2), which reflects the functional conservation of these DDR genes in fungi [ 40- 44].
DoT had the best performance in SPO SCE prediction because, relative to that in bacteria, gene essentiality is more conserved through the function of protein domains or domain combinations rather than through the conservation of the entire genes in fungi [ 28].
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