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In tropical America, coffee is traditionally grown under a diverse canopy of overstory shade trees, which enhances the quality of the coffee farm as a conservation matrix and supports a broad spectrum of pollinators that increase fruit set per bush [2 4].
Thus, the genome conservation matrix "expresses the conservation of both sequence and gene content between two genomes" [ 36].
Principal component analysis of the conservation matrix was then performed using the Matlab Statistics toolbox (The Mathworks Inc., MA, USA).
A conservation matrix was obtained (Table 6) from the genome-wide comparison through the pairwise alignment of 'Lovell' reference genome sequence and the three genotypes studied.
For example, at position -2 42% of the domains contained a serine, thus the conservation matrix was filled with 0.42 for all domains with serine at this position.
8. Conserved k-mers – This method for constructing a k-mer conservation matrix is based on output generated by the PhastCons algorithm [ 41, 65].
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Then the position conservation correlation matrix and position mutation correlation matrix is constructed from the amino acid position mutative equation.
The UVs were further classified as high risk or low risk based on five methods: allele frequency, Polyphen algorithm, sequence conservation, Grantham matrix scores, and a combination of the Grantham matrix score and sequence conservation.
The position conservation correlation matrix can be derived from the reduced position frequency matrix FM×L.
The position conservation correlation matrix R(con)L×L of PDZ is graphically shown in Fig. 2. The matrix R(con)L×L is symmetric to the diagonal line.
It is difficult to dig out detailed information from the position conservation correlation matrix R(con)L×L of PDZ database, because too many conservative positions complicate the analysis.
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