The upper six rows of the table show brain expression patterns, subcellular localisation, evolutionary conservation, and potential molecular function of known mental retardation genes.
Midline localisation, periumbilical or poorly localised 2.
However, several lines of evidence presented here argue for ENSDARG00000090815 as the relevant orthologue: (1) the chromosomal localisation of ENSDARG00000090815 retains the evolutionary conservation of tandem pairs of genes encoding KCNJ channels.
The experimental data demonstrate conservation of MACIT molecular and cellular properties and tissue localisations in the invertebrate, C. elegans.
Comparison of the amino acid sequences of PDX1 and PDX2 across vertebrates shows conservation of the Pbx-interacting motif, DNA-binding domain and nuclear localisation signal but not of known transactivation domains and the PCIF1-interaction domain [ 119– 119].
Given the evolutionary conservation of physical interaction between these two proteins, we next addressed the localisation patterns of hMsd1/SSX2IP upon Wdr8 depletion using siRNA oligonucleotides and vice versa.
Given the high degree of conservation between the human and fly Bug22 orthologs (92%) and the ciliary localisation of hBug22 in hTERT-RPE1 cells (Fig. 1D and Ishikawa et al., 2012), we decided to test whether primary cilium tubulin PTMs were also dependent on Bug22.
According to observations in Picea meyeri pollen tubes (Liu et al., 2009), sterol depletion also destroyed the localisation of ordered membrane domains in tobacco pollen tube PM, suggesting a conservation mechanism maintaining PM asymmetry in the male gametophyte through plant evolution.
The localisation of type I marker on this map has provided some evidences of conservation of syntenies between zebrafish and rainbow trout, though it is not the rule.
Due to the perfect sequence conservation between the CLE41/44 and CLE209/210 peptides, we sought to verify experimentally whether the phloem-specific expression localisation of the CLE41/44 genes [ 9] was also conserved in P. radiata.
In addition, the efficiency of a di-lysine motif for ER localisation of transmembrane proteins in cells has also been described in mammals, yeasts and plants [ 12- 15], suggesting a conservation of the machinery.
