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Figure 3 shows the resulting conservation estimates.
Consequently, a substantial fraction of genomic sites should suffer from misleading conservation estimates.
As such, sitewise as well as regional conservation estimates may not always perfectly reflect the presence of functional selection.
As we have shown, conservation estimates are highly asymmetric with regard to the preferred base and typically vary over time.
Purifying selection of the same strength can thus give rise to very different conservation estimates depending on the particular scenario.
Moreover, conservation estimates depend also on the orientation of selection relative to mutational biases and can vary over time.
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The level of conservation, estimated by the phastCons score [ 70] and phyloP [ 71], had weakly positive associations with level of activity in the transient transfection assay (correlation coefficients from linear regression R = +0.173 and R = +0.015, respectively, Additional file 2: Figure S14B).
The final conservation score was then taken to be the minimum of these correlation scores, as a conservative estimate of conservation.
The loggerhead turtle, which the government lists as threatened, is far more productive: it will dig about 60,000 nests this year, the Caribbean Conservation Corporation estimates.
Oceana, a marine conservation group, estimates that since 2000, the European Union has provided 8 billion euros of subsidies to the industry, with national governments led by Ireland, Spain, Italy and France topping that up with an additional total of 4.9 billion euros.
BirdLife, the international partnership of bird conservation groups, estimates that between 2.5 and 3 million songbirds are now annually trapped across the island as a whole, with the greatest number on the UK bases, especially Dhekelia, where hundreds of thousands are believed to be caught.
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