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Using the above criteria, we obtained the human, mouse, dog and rat (HMDR) conservation dataset, and its members represented conserved candidate hairpins having orthologs in human, mouse, dog and rat.
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At the exon level, we observed that 97 of 120 human exons (Table 7) were conserved in bovine, indicating a high level of conservation in this dataset across both the species.
The features used in their prediction included the closeness feature, solvent accessible surface, and sequence conservation using a dataset of 178 enzymes.
Even though conservation across independent datasets was much reduced for SBP and HR, the number of significant between-projects correlations was higher than expected by chance for both traits.
When this study was essentially completed, two studies appeared that addressed the problem of exon conservation in vertebrate datasets [ 9, 10].
The ChIP-seq datasets Conservation of binding sites amongst vertebrates was performed using the Cistrome Analysis Pipeline (http://cistrome.dfci.harvard.edu/ap).edu/ap
This coverage is greater than the ones obtained for other phylomes such as the Schistosoma mansoni (70%%) [ 24] or the pea aphid Acyrthosiphon pisum (67%%) [ 26], thereby underscoring the high quality and sequence conservation of the datasets.
Since we anticipate that genes highly conserved in expression response in one condition could diverge significantly in another condition, so we investigated expression conservation using a dataset-specific approach.
Then, for each of the lysine residues on those sequences the degree of conservation in the entire dataset was determined.
For S. cerevisiae, we also tested the performance of distance measures and their influence on conservation to a second dataset [ 20] that contained up to 300 experimental conditions.
Remarkably, many of these same categories were enriched in the mouse dataset, showing conservation of function between the two species among trans ceQTLs, particularly ion related categories.
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