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A variety of probes, including positive, negative, and specific, were first developed by sequencing and consequent sequence analysis of large subunit rDNA D1 D2 from target species and used for specificity test by blot hybridization.
Hence, it appears that lidocaine and riluzole, both myocardial INaP blockers, significantly reduced the extent of myocardial damage from ischaemia by reducing the [Na+]i overload and consequent sequence of events leading to [Ca2+]i loading and cell damage.
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Two hundred subregions exhibiting subchondral BMLs from 63 knees of 42 subjects in the primary reading were randomly chosen for the consequent direct sequence comparison.
This observation is consistent with the older age of these populations with the consequent accumulation of sequence variants over a greater number of generations [29], [30].
This is due to the lower hybridization efficiency of probes consequent to increased sequence divergence.
Towards the end of this period the game changed considerably: first with the introduction of X. tropicalis into the equation, and then with the advent of large-scale EST projects and their consequent full-length sequencing programs, although these were by no means independent events.
Because of the advent of high-throughput sequencing and the consequent reduction in the cost of sequencing, many organisms have been completely sequenced and most of their genes identified.
Anecdotal evidence of the presence of a further extension of mRNA 5' end sequence, with consequent correction of the previously accepted predicted product, was randomly found for single genes.
A condor fibroblast cell line was utilized for mRNA extraction and consequent generation of cDNA sequences at Washington University Genome Sequencing Center using the 454 sequencing technology [ 48].
Technological enhancements and consequent reduction of time in sequence capture have enabled sequencing of several chloroplast genomes recently.
The population then peaked and plateaued (median population size range, 317.1 325.0) during 1985–1995, possibly due to advances in nucleotide sequencing technology and the consequent increase in the number of sequences reported worldwide.
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