We next assessed the consequences of the structural and biochemical alterations in mutant Rab7 on membrane targeting and exchange dynamics.
One consequence of the structural reorganization that occurs on substrate binding is to place trigger loop residues proximal to substrate groups important for substrate recognition and phosphodiester bond formation, suggesting that the trigger loop may have a direct function in catalysis.
The resulting PCR fragment contained the amino acid residue sequence M AGSWLRDIWDWICEVLSDFKTWLKAKLMP T M, which incorporates a start Met, as well as two codon sequence changes (Ser1Ala and Gln31Thr, underlined) expected to behave as silent mutations with no anticipated consequence on the structural folding of the amphipathic helix domain contained from residues 5 25.
The daughter crystals, rotated by 37°, are produced on the edges of the parent crystals via an epitaxial growth that is a direct consequence of the structural disorder.
Based on the observations gathered from the tests, some aspects of the design of "simple" joints are briefly discussed, and their consequences on the global structural analysis are illustrated using three worked examples; due to sensitivity to buckling, it appears that the usual assumption of pinned joints may be unconservative, however in very specific cases.
For safety purposes, it is important to understand and preview the consequences of fire on the structural integrity of concrete.
In the small fleet (only 16 aircraft were ever delivered to the airlines, the last in 1979) there were 57 tire bursts or deflations, of which 12 had "structural consequences on the wings and/or the tanks, of which six led to penetration of the tanks," according to a report released in December by the B.E.A. (Bureau Enquêtes-Accidents), a French government agency.
By using anti-ICAM-1 monoclonal antibodies in rats and ICAM-1 transgenic mice, Kelly and colleagues have shown that this adhesion molecule plays an early and important pathogenetic role in renal ischemia-reperfusion, via neutrophil infiltration, with both functional and structural consequences on the kidney [ 28, 29].
Modern requirements on constructions impose that proper design strategies must be adopted in order to obtain a robust structure: in this sense, consequence-based design focuses the attention on the structural response to damage.
