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We report here that the mTORC1-mediated consequences on cell cycle and cell size are separable and do not involve effects on mTORC2 activity.
Functional consequences on cell cycle and cell motility were determined by fluorescence activated cell sorting (FACS) analysis and cell migration assay respectively.
Making use of non-transformed, non-immortalized primary human fibroblasts we found that (i) the mTORC1-mediated consequences on cell cycle and cell size are separable and do not involve effects on mTORC2 activity; (ii) In addition, we report here for the first time that mTORC2 is a potent regulator of mammalian cell size and cell cycle via a mechanism involving Akt/TSC2/Rheb.
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The reduction of BRCA1 foci did not appear to be a consequence of changes in cell cycle, as the silencing of Cav-1 had no effect on cell cycle distribution (Fig. 6).
We have facilitated dissemination and community access to these data on the proteomic consequences of cell cycle arrest by depositing the data in multiple repositories targeted for different user audiences.
Furthermore, by flow cytometry the consequences of HNF6 expression on cell cycle regulation in transfected cells was studied.
The analysis of genes associated with cell functions provides new insight into consequences of FGFR3 mutations on cell cycle regulation, onset of pre-hypertrophic differentiation, concomitant metabolism changes and adhesion.
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To determine whether predicted local changes in cellular processes have functional consequences, we first focused on cell cycle parameters.
Predisposition to breast and extrauterine Müllerian carcinomas in BRCA1 mutation carriers is due to a combination of cell-autonomous consequences of BRCA1 inactivation on cell cycle homeostasis superimposed on cell-nonautonomous hormonal factors magnified by the effects of BRCA1 mutations on hormonal changes associated with the menstrual cycle.
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