Despite this stringent cut-off, false positive errors were still detected, mostly as a consequence of the sequence specific error profiles of both sequencers (Table 2, [ 21, 38, 39]).
3' termini of genes are AT-rich, suggesting that the depletion of nucleosomes seen in MNase dependent experiments are likely to be the consequence of the sequence preferences of MNase.
This cis-regulatory code is a consequence of the sequence specificity of transcription factors (TFs) and their interactions with other TFs, nucleosomes and other chromatin-associated proteins.
Nevertheless, sequence variation among genomes was mostly a consequence of the sequence variation incorporated in introns and intergenic regions, while genome size was mostly a function of intron density and to some extent the size variation of certain intergenic regions.
The sequences defining the following subtypes in the previous nomenclature were not included in the new nomenclature as a consequence of the sequence validation: P1.2a, P1.2d, P1.5b, P1.10h, P1.10i, P1.10j, P1.18b, P1.19c, P1.24, P1.29.
This value also showed a much smaller change for the two alternate reading frames (1.22 for both), suggesting that it may be a consequence of the sequence composition and not codon dependent.
Although this DNA can exhibit DNA bending in vitro[ 44] (and can be packaged into nucleosomes [ 18, 20, 28]) the structural consequences of this sequence characteristic for the organization of chromatin are presently unknown.
While the consequences of this sequence variability are unknown, it could reflect functional attributes of corresponding translation products, such as the diversity of ligand binding demonstrated in some repeat proteins with analogous characteristics [ 5, 34].
Paracchini et al. studied the functional consequences of these sequence variants in lymphoblastoid cell lines [ 9].
The potential consequences of this sequence of events or practices for organisations and individuals with diabetes are significant.
