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To test this hypothesis, we constructed a set of R. palmatum BAS site-directed mutants (L132G, L132A, L132S, L132C, L132T, L132F, L132Y, L132W and L132P), and investigated the mechanistic consequences of the point mutations.
To assess the functional consequences of the point mutations, pri-miRNAs and mutant versions of each miRNA were co-transfected with their corresponding siRNA (si) and miRNA (mi) targets (see Materials and Methods).
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As a consequence of the point mutation in the YMDD motif of the hepatitis B virus (HBV) polymerase gene, lamivudine-resistant mutants have been reported in chronic hepatitis B patients who underwent lamivudine therapy.
In direct contrast, the phenotype in the model characterised here, with a greater impact on cones than on rods, is likely to be a direct consequence of the point mutation in GCAP1.
She wanted to leave, she wanted to lie alone, face-down on her bed and savour the vile piquancy of the moment, and go back down the lines of branching consequences to the point before the destruction began.
We examine the consequences of this point of view on the architecture process.
The subsequent sections on substance and the ego point to further consequences of the empiricist point of view adopted by the mainstream neutral monists.
Ian Bloom, one of the sharpest legal brains in the country, says: "Any team is bound to protest to whatever court it can find to hear the appeal - especially if the consequence of the points deduction is relegation or missing a European place".
Therefore, the consequences of the inflection point that accompanies the abrupt change in the velocity are mitigated and a reliable time varying Doppler shift is obtained.
These findings provide basic knowledge on the efficacy of selected corticosteroids to treat articular cartilage damage and prevent of the same via use of these drugs in combination; the consequences of the treatment point to the possibility for application of these mechanical effects in the clinical environment.
The "merit" of actions, he says in Book II of TMS, depends on their consequences, even if their propriety is independent of consequences; the point, for him, is just that these are two different elements of moral judgment and the first is of greater importance than the second (188).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com