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To assess the potential genotoxic risk of conventional or self-inactivating (SIN) γ-RV and lentiviral (LV) vectors independently from the biological consequences of the insertion event, we developed a quantitative assay based on real-time reverse transcriptase PCR on low-density arrays to evaluate alterations of gene expression in individual primary T-cell clones.
To further study the consequences of the insertion of introns within UTRs, we also retrieved the paralogous set of genes available on the MSU site at http://rice.plantbiology.msu.edu/annotation_pseudo_ortho.shtml.shtml
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Most of their differences are localized in the HPRs of these genomes, and these are primarily the consequence of the insertion, deletion, or replacement of ORFans.
PFO membrane insertion can be monitored by a change in fluorescence (as a consequence of the insertion of several tryptophan residues into the membrane).
Intergenic regions were on average 1.6 times longer in S. latifolia than in S. vulgaris, mainly as a consequence of the insertion of transposable elements.
It is plausible that the advantages in the ultrasound group with respect to proper guidewire insertion can not only be seen as a consequence of ultrasound usage as well as but also as a consequence of the insertion site.
Two alternative adaptations have been proposed for Methanosarcina spp. and the bacteria Acetohalobium arabaticum to minimize proteome alteration in consequence of the insertion of Pyl on the stop codons normally intended to stop the translation [ 77].
These differences could be a consequence of the insertion of two strong promoters at MATa2 and suggest that controlled measurements of growth rates (or other traits of interest) are required for strains containing the RFP cassette.
The structural consequences of this insertion are not well understood at present.
In the present study, we first investigated the conformational properties of V22-SH3 assessedssed the consequence of the insertions of other amino acids at the same position by replacing Val22 with Ala, Asp or Arg.
Although the kinetic consequences of the Asp insertion are well-established, how it impacts the structure of PBP2 is unknown.
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